College biology 102/103 lab 5: meiosis

 

·         Complete this Lab 5Answer Sheet electronically and submit it via the Assignments Folder by the date listed intheCourse Schedule (under Syllabus).

·         To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.

·         Save your Lab 5Answer Sheet in the following format:  LastName_Lab5 (e.g., Smith_Lab5).

·         You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.

 

By submitting this assignment for credit, you are affirming that you have completed the answers yourself to the best of your ability, that you have used your own words to answer the questions and have not engaged in any acts of academic dishonesty to answer the questions below.  Acts of academic dishonesty include submitting these questions to any online website or service in order to obtain the answers as well as obtaining the help of any other individual for the purpose of answering the questions.

 

 

 

 

 

 

Experiment 1: Following Chromosomal DNA Movement through Meiosis

Part 1 – Meiotic Division Bead Pictures without Crossing Over – You must provide pictures of your bead manipulations at each stage.

Prophase I

 

Metaphase I

 

Anaphase I

 

Telophase I

 

Prophase II

 

Metaphase II

 

Anaphase II

 

Telophase II

 

Cytokinesis

 

 

 

Part 2: Meiotic Division Beads Diagram with Crossing Over – You must provide pictures of your bead manipulations at each stage.

Prophase I

 

Metaphase I

 

Anaphase I

 

Telophase I

 

Prophase II

 

Metaphase II

 

Anaphase II

 

Telophase II

 

Cytokinesis

 

 

 

 

 

Post-Lab Questions

 

 

1.    Were any of your daughter cells genetically identical at the end of Part I above?  What about Part II?  What does this tell you about the importance of crossing over?

      

 

2.    What must occur during interphase for meiosis to be successful?

 

 

 

3.    When does the diploid number become haploid during meiosis?

 

4.    Why did you use non-sister chromatids to demonstrate crossing over?

 

5.    What combinations of alleles could result from a crossover between a chromosome having genes BD and a chromosome having genes bd?

 

 

 

 

6.    Blue whales have 44 chromosomes in every cell. Determine how many chromosomes you would expect to find in the following:

 

Sperm Cell:

Egg Cell:           

Daughter Cell from Meiosis I:

Daughter Cell from Meiosis II:

 

 

7.    What is the difference between a chromosomal mutation and a gene mutation?

 

8.    Diagram what would happen if sexual reproduction took place for four generations using diploid (2n) cells.

 

 

 

 

 

 

 

 

 

Experiment 2: The Importance of Cell Cycle Control

 

Data – Enter your karyotype abnormalities below.  Explain the abnormality in terms of chromosome number and structure.  Do not post pictures of the karyotypes themselves.

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Post-Lab Questions

 

1.    What is your hypothesis as to what differences you might observe when comparing the karyotypes of human cells which experience normal cell cycle control versus cancerous cells (which experience abnormal, or a lack of, cell cycle control)?

 

 

 

2.    Did your results confirm or disprove your hypothesis?  Why or why not?  Be specific!

                   

 

3.    Suppose a person developed a mutation in a skin cell which results in cancer, but the cancer was effectively treated with a cocktail of cancer-fighting techniques. Is it possible for this person’s offspring to inherit this cancer-causing mutation? Why or why not?  Be specific!

 

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